Gat1 (Gaba:Na+:Cl−) Cotransport Function

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Gat1 (Gaba:Na+:Cl−) Cotransport Function

Neurotransmitter transporters are reported to mediate transmembrane ion movements that are poorly coupled to neurotransmitter transport and to exhibit complex "channel-like" behaviors that challenge the classical "alternating access" transport model. To test alternative models, and to develop an improved model for the Na+- and Cl--dependent gamma-aminobutyric acid (GABA) transporter, GAT1, we e...

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Cotransport Function Database Reconstruction with an Alternating Access Model

We have developed an alternating access transport model that accounts well for GAT1 (GABA:Na 1 : Cl 2 ) cotransport function in Xenopus oocyte membranes. To do so, many alternative models were fitted to a database on GAT1 function, and discrepancies were analyzed. The model assumes that GAT1 exists predominantly in two states, E in and E out . In the E in state, one chloride and two sodium ions...

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Enhanced learning and memory in GAT1 heterozygous mice.

γ-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the central nervous system. The termination of GABA transmission is through the action of a family of membrane proteins, called GABA transporters (GAT1-4). It is well established that GABA system is involved in the modulation of memory. Our previous study showed that homozygous GAT1(-/-) mice exhibited impaired hippocampus-d...

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Sodium-potassium-chloride cotransport.

Obligatory, coupled cotransport of Na(+), K(+), and Cl(-) by cell membranes has been reported in nearly every animal cell type. This review examines the current status of our knowledge about this ion transport mechanism. Two isoforms of the Na(+)-K(+)-Cl(-) cotransporter (NKCC) protein (approximately 120-130 kDa, unglycosylated) are currently known. One isoform (NKCC2) has at least three altern...

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Coordinate modulation of Na-K-2Cl cotransport and K-Cl cotransport by cell volume and chloride.

Na-K-2Cl cotransporter (NKCC) and K-Cl cotransporter (KCC) play key roles in cell volume regulation and epithelial Cl(-) transport. Reductions in either cell volume or cytosolic Cl(-) concentration ([Cl(-)](i)) stimulate a corrective uptake of KCl and water via NKCC, whereas cell swelling triggers KCl loss via KCC. The dependence of these transporters on volume and [Cl(-)](i) was evaluated in m...

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ژورنال

عنوان ژورنال: Journal of General Physiology

سال: 1999

ISSN: 0022-1295,1540-7748

DOI: 10.1085/jgp.114.3.459